{"id":536,"date":"2025-07-02T19:36:28","date_gmt":"2025-07-02T19:36:28","guid":{"rendered":"https:\/\/staging-3.com\/university\/?page_id=536"},"modified":"2025-07-02T20:00:56","modified_gmt":"2025-07-02T20:00:56","slug":"asymmetrical-division","status":"publish","type":"page","link":"https:\/\/staging-3.com\/university\/asymmetrical-division\/","title":{"rendered":"Asymmetrical Division"},"content":{"rendered":"\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
<\/div>\n\t\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t

know us better<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t

Asymmetrical Division<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t

<\/h2><\/div>

We are interested in characterizing the specific cell lineages involved in the development of the mammalian brain. \u00a0The mammalian forebrain contains neural stem cells that can proliferate to produce cells with multiple cellular and spatial phenotypes and that are capable of long term self-renewal (over the lifetime of the animal). \u00a0In studying the earliest origin of these neural stem cells in the embryo, we have been led by our data to test the hypothesis that the default state for mouse embryonic stem cells is to become neural stem cells. \u00a0Mouse embryonic stem (ES) cells are lines of pluripotent early blastocyst cells that can give rise in vivo to entire and completely viable mice. \u00a0In vitro in the presence of serum these ES cells will form aggregates called embryoid bodies containing cells that differentiate into blood, neural, muscle and other cell types \u00a0In contrast to these differentiating ES cell aggregates (embryoid bodies), however, we found that when cultured under serum-free, low cell density conditions, a small (but quantitatively reliable) percentage (0.2%) of single ES cells proliferate in the presence of leukemia inhibitory factor (LIF) to form floating sphere colonies of cells that express the neuroepithelial marker nestin, but no longer express the ES cell markers \u00a0Oct4 and SSEA1.<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t
\n\t\t\t\t\t

Related Publications:<\/h2><\/div>

Karpowicz, P., Pellikka, M., Chea, E., Godt, D., Tepass, U., and van der Kooy, D. The germline stem cells of Drosophila melanogaster partition DNA non-randomly. European Journal of Cell Biology. 88 (2009) 397-408.\u00a0(karpowicz...2009)

\u200d<\/a>Karpowicz P, Morshead C, Kam A, Jervis E, Ramunas J, Cheng V, van der Kooy D. Support for the immortal strand hypothesis: neural stem cells partition DNA asymmetrically in vitro. Journal of Cell Biology 170 (2005) 721-32.\u00a0(karpowicz...2005...)<\/a><\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

\n\t\t\t\t
\n\t\t\t\t\t

Photo Credit: Justin Belair-Hickey<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t

VDK Lab<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t
<\/i><\/div><\/div>
\n

Address<\/h2>

160 College Street, 11th floor\nDonnelly Centre for Cellular and Biomolecular Research\nUniversity of Toronto<\/p>\n <\/div>\n \n <\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t
<\/i><\/div><\/div>
\n

Contact<\/h2>

+1 (416) 978-4539<\/p>\n <\/div>\n \n <\/div><\/a><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t
<\/i><\/div><\/div>
\n

Management Business<\/h2>

brenda.coles@utoronto.ca<\/p>\n <\/div>\n \n <\/div><\/a><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

know us better Asymmetrical Division We are interested in characterizing the specific cell lineages involved in the development of the mammalian brain. \u00a0The mammalian forebrain contains neural stem cells that can proliferate to produce cells with multiple cellular and spatial phenotypes and that are capable of long term self-renewal (over the lifetime of the animal). […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"footnotes":""},"class_list":["post-536","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/staging-3.com\/university\/wp-json\/wp\/v2\/pages\/536","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/staging-3.com\/university\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/staging-3.com\/university\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/staging-3.com\/university\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/staging-3.com\/university\/wp-json\/wp\/v2\/comments?post=536"}],"version-history":[{"count":10,"href":"https:\/\/staging-3.com\/university\/wp-json\/wp\/v2\/pages\/536\/revisions"}],"predecessor-version":[{"id":575,"href":"https:\/\/staging-3.com\/university\/wp-json\/wp\/v2\/pages\/536\/revisions\/575"}],"wp:attachment":[{"href":"https:\/\/staging-3.com\/university\/wp-json\/wp\/v2\/media?parent=536"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}